Main sol. 678
Internal Solution-ID: S1428
Equipment needed: Hungate tubes
Autoclave
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NH4Cl | 1.0 | g | 1 | 18.695 | |
| MgCl2 x 6 H2O | 1.0 | g | 1 | 4.919 | |
| CaCl2 x 2 H2O | 0.4 | g | 0.4 | 2.721 | |
| K2HPO4 x 3 H2O | 0.4 | g | 0.4 | 2.297 | |
| Modified Wolin's mineral solution | 10.0 | ml | - | - | |
|
Trypticase peptone
(BD BBL) |
2.0 | g | 2 | - | |
|
Yeast extract
(OXOID) |
2.0 | g | 2 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | - | |
| NaHCO3 | 8.4 | g | 8.4 | 99.992 | |
| Gelatine | 3.0 | g | 3 | - | |
| Na2S x 9 H2O | 0.5 | g | 0.5 | 2.082 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve ingredients (except bicarbonate, gelatine and sulfide), then sparge medium with 100% CO2 gas for 30 - 45 min to make it anoxic. Add and dissolve bicarbonate and equilibrate the medium with the CO2 gas to achieve a pH of 7.0. Dispense the medium under 100% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add gelatine and sulfide from sterile anoxic stock solutions autoclaved under 100% N2 gas. Prior to inoculation check pH of complete medium and adjust to 7.0, if necessary. | |||||
Media with this solution
| 678 | COPROTHERMOBACTER (CP) MEDIUM |