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Main sol. 1076b

Solution-ID: S143

Equipment needed: Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.70 g 3.535 -
2 2.00 g 10.101 -
3
(BD)
1.00 g 5.051 -
4 , adjust if required 130.00 ml 655.388 36379.5
5
(fish sperm; SERVA)
0.04 g 0.202 -
6
(10x concentrated; GIBCO)
10.00 ml - -
7
(BD; 2%, autoclaved)
20.00 ml - -
8
(heat-inactivated)
17.00 ml - -
9
(heat-inactivated)
17.00 ml - -
10 , either
(50% aqueous solution)
2.00 ml - -
11 , or
(50% aqueous solution)
2.00 ml - -
12
optional
0.40 g 2.02 33.639
Instructions:
  1. For 200 ml of medium add: #1-5
  2. Adjust pH to 7.4; autoclave at 121°C for 15 minutes.
  3. Add aseptically: #6-9
  4. As energy source add either #10 or #11
  5. For Ureaplasma add #12 instead of Glucose or Arginine
  6. For agar plates use only 126 ml of distilled water and add 2 g of agar . Medium containing agar should be placed at 55°C before adding supplements.
1076b SP4-Z MEDIUM