Main sol. 688
Internal Solution-ID: S1436
Equipment needed: Hungate tubes
Autoclave
Filter
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 12.00 | g | 11.869 | 203.105 | |
| MgSO4 x 7 H2O | 0.50 | g | 0.495 | 2.007 | |
|
PIPES
(SIGMA) |
3.40 | g | 3.363 | 11.122 | |
| KCl | 2.00 | g | 1.978 | 26.536 | |
| NH4Cl | 0.10 | g | 0.099 | 1.849 | |
| CaCl2 x 2 H2O | 0.03 | g | 0.03 | 0.202 | |
| K2HPO4 | 0.02 | g | 0.02 | 0.114 | |
| Modified Wolin's mineral solution | 10.00 | ml | - | - | |
| Yeast extract | 0.50 | g | 0.495 | - | |
|
Peptone
(meat, peptic) |
1.00 | g | 0.989 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 4.9e-4 | 0.002 | |
| Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
| Na2S x 9 H2O | 0.50 | g | 0.495 | 2.059 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except vitamins and sulfide), adjust pH to 7.0 and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add sulfide from a sterile anoxic stock solution autoclaved under100% N2 gas. Vitamins are prepared under 100 N2 gas and sterilized by filtration. Adjust pH of complete medium to 7.0, if necessary. | |||||
Media with this solution
| 688 | PSEUDOTHERMOTOGA SUBTERRANEA MEDIUM |