Main sol. 689
Internal Solution-ID: S1437
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
KCl | 0.33 | g | 0.329 | 4.413 | |
MgCl2 x 6 H2O | 6.00 | g | 5.982 | 29.424 | |
MgSO4 x 7 H2O | 1.00 | g | 0.997 | 4.045 | |
NH4Cl | 0.25 | g | 0.249 | 4.66 | |
CaCl2 x 2 H2O | 0.14 | g | 0.14 | 0.949 | |
K2HPO4 | 0.14 | g | 0.14 | 0.801 | |
NaCl | 18.00 | g | 17.946 | 307.087 | |
Fe(NH4)2(SO4)2 x 6 H2O
(0.1% w/v) |
2.00 | ml | 0.002 | - | |
Yeast extract
(Difco) |
1.00 | g | 0.997 | - | |
Resazurin | 1.00 | mg | 1.0e-3 | 0.004 | |
L-Cysteine HCl x H2O | 0.50 | g | 0.499 | 2.838 | |
NaHCO3 | 2.00 | g | 1.994 | 23.736 | |
Glucose | 10.00 | g | 9.97 | 55.34 | |
Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve ingredients except cysteine, bicarbonate, glucose and vitamins, then sparge medium with 100% N2 gas to make it anoxic. Add cysteine and sodium bicarbonate, then adjust pH to 7.5. Dispense medium in anoxic Hungate-type tubes under 100% N2 gas and autoclave. A precipitate forms which will redissolve slowly over about 3 days. Before use inject separately sterile glucose solution (20% w/v) and vitamins prepared under 100% N2 gas atmosphere. Vitamins should be sterilized by filtration. Check medium pH and adjust pH to 7.6, if necessary. |
689 | ACE MEDIUM |