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Main sol. 691

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Internal Solution-ID: S1439

Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 1.40 g 1.396 10.257
NH4Cl 0.50 g 0.499 9.32
MgCl2 x 6 H2O 0.20 g 0.199 0.981
CaCl2 x 2 H2O 0.15 g 0.15 1.017
Trace element solution SL-10 1.00 ml - -
Selenite-tungstate solution 1.00 ml - -
NaHCO3 1.50 g 1.496 17.802
Na2-DL-malate 1.60 g 1.595 8.959
Wolin's vitamin solution (10x) 1.00 ml - -
Distilled water 1000.00 ml - -
1 Dissolve ingredients except vitamins, bicarbonate and malate. Adjust pH to 6.5, dispense medium in culture vessels and autoclave. Prior to inoculation add vitamins, bicarbonate and malate from stock solutions sterilized by filtration. Adjust pH of complete medium to 6.7 - 6.9.
691 CreDm1 MEDIUM