Main sol. 701
Internal Solution-ID: S1452
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NH4Cl | 1.0 | g | 1 | 18.695 | |
MgCl2 x 6 H2O | 7.0 | g | 7 | 34.431 | |
MgSO4 x 7 H2O | 9.6 | g | 9.6 | 38.949 | |
CaCl2 x 2 H2O | 0.5 | g | 0.5 | 3.401 | |
KCl | 3.8 | g | 3.8 | 50.973 | |
K2HPO4 x 3 H2O | 0.4 | g | 0.4 | 2.297 | |
NaCl | 10.0 | g | 10 | 171.116 | |
NaHCO3 | 3.0 | g | 3 | 35.711 | |
Na2CO3 | 1.0 | g | 1 | 9.435 | |
Na2S x 9 H2O | 0.5 | g | 0.5 | 2.082 | |
Yeast extract | 1.0 | g | 1 | - | |
L-Cysteine | 0.5 | g | 0.5 | 4.127 | |
Resazurin | 1.0 | mg | 1.0e-3 | 0.004 | |
Substrate
(glucose, chitin etc.) |
5.0 | g | 5 | - | |
Modified Wolin's mineral solution II | 1.0 | ml | - | - | |
Distilled water | 1000.0 | ml | - | - | |
1 pH 7.2 | |||||
2 Prepare the medium without the Na2S, Na2CO3, NaHCO3, L-cysteine and substrate. Boil the medium and cool under a stream of nitrogen. Add the Na2CO3 and NaHCO3 . Adjust the pH to 7.2. If chitin is used as the substrate it must be added before autoclaving. Autoclave the medium and add the Na2S (from a sterile, neutralized stock solution prepared under nitrogen), L-cysteine (from a stock solution prepared under nitrogen), and the glucose or N-acetylglucosamine (if used as substrate) from sterile stock solution prepared under nitrogen. Check the final pH. |
701 | HALOANAEROBACTER CHITINOVORANS MEDIUM |