Main sol. 705
Internal Solution-ID: S1456
Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Ferric citrate monohydrate | 5.3 | g | 5.146 | 19.568 | |
| NaCl | 5.8 | g | 5.631 | 96.356 | |
| NH4Cl | 1.0 | g | 0.971 | 18.151 | |
| K2HPO4 x 3 H2O | 0.4 | g | 0.388 | 2.23 | |
| MgCl2 x 6 H2O | 1.0 | g | 0.971 | 4.776 | |
|
Yeast extract
(OXOID) |
2.0 | g | 1.942 | - | |
|
Trypticase peptone
(BD BBL) |
2.0 | g | 1.942 | - | |
| Trace element solution | 10.0 | ml | - | - | |
| Na-acetate | 0.8 | g | 0.777 | 9.468 | |
| Na-lactate | 1.2 | g | 1.165 | 10.397 | |
|
2-Mercaptoethanesulfonic acid
(coenzyme M) |
0.5 | g | 0.485 | 3.414 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 First, dissolve ferric citrate in water by heating and adjust to pH 7.5 with NaOH, then add other medium ingredients except acetate, lactate, and coenzyme M. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense under 80% N2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add acetate, lactate, and coenzyme M from sterile anoxic stock solutions prepared under 100% N2 gas. | |||||
| 2 Prior to inoculation the pH of the complete medium should be checked and adjusted to 7.5, if necessary. | |||||
Media with this solution
| 705 | BACILLUS INFERNUS MEDIUM |