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Main sol. 709

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Internal Solution-ID: S1459

Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
MgSO4 x 7 H2O 0.50 g 0.5 2.029
(NH4)2SO4 0.40 g 0.4 3.027
K2HPO4 0.20 g 0.2 1.148
KCl 0.10 g 0.1 1.341
FeSO4 x 7 H2O 0.01 g 0.01 0.036
Yeast extract 0.25 g 0.25 -
Distilled water 1000.00 ml - -
1 Dissolve ingredients except yeast extract and adjust pH to 2.0 with H2SO4. After autoclaving add yeast extract from a sterile stock solution.
2 Note: For autotrophic growth omit yeast extract and add to the medium 13.90 g/l FeSO4 x 7 H2O. Adjust medium pH to 1.7 with H2SO4 prior to autoclaving.
709 ACIDIMICROBIUM MEDIUM