Main sol. 711
Internal Solution-ID: S1461
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NH4Cl | 1.00 | g | 0.997 | 18.639 | |
K2HPO4 | 0.30 | g | 0.299 | 1.717 | |
KH2PO4 | 0.30 | g | 0.299 | 2.198 | |
MgCl2 x 6 H2O | 0.20 | g | 0.199 | 0.981 | |
CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.678 | |
KCl | 0.10 | g | 0.1 | 1.337 | |
NaCl | 0.60 | g | 0.598 | 10.236 | |
Yeast extract | 0.50 | g | 0.499 | - | |
Na-acetate x 3 H2O | 0.50 | g | 0.499 | - | |
Trace element solution SL-10 | 1.50 | ml | - | - | |
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | 0.002 | |
Na2CO3 | 1.50 | g | 1.496 | 14.11 | |
3,4,5-Trimethoxycinnamic acid
(TMC) |
1.19 | g | 1.186 | 4.98 | |
Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
L-Cysteine HCl x H2O | 0.30 | g | 0.299 | 1.703 | |
Na2S x 9 H2O | 0.30 | g | 0.299 | 1.245 | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve ingredients (except carbonate, vitamins, TMC and reducing agents) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add vitamins, TMC, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. The vitamin solution should be sterilized by filtration. The stock solution of TMC (6% w/v) should be neutralized with NaOH prior to sterilization by filtration. Adjust pH of complete medium to 7.0, if necessary. |
711 | SPOROBACTER MEDIUM |