Main sol. 712
Internal Solution-ID: S1463
Equipment needed: Filter
Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| K2HPO4 | 0.23 | g | 0.23 | 1.319 | |
| KH2PO4 | 0.23 | g | 0.23 | 1.688 | |
| NaCl | 0.45 | g | 0.45 | 7.693 | |
| (NH4)2SO4 | 0.45 | g | 0.45 | 3.402 | |
| CaCl2 x 2 H2O | 0.06 | g | 0.06 | 0.408 | |
| MgSO4 x 7 H2O | 0.09 | g | 0.09 | 0.365 | |
| Yeast extract | 5.00 | g | 4.995 | - | |
| Indigocarmine | 5.00 | mg | 0.005 | 0.011 | |
| NaHCO3 | 6.40 | g | 6.394 | 76.108 | |
| D-Glucose | 5.00 | g | 4.995 | 27.725 | |
| Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
| L-Cysteine HCl x H2O | 0.30 | g | 0.3 | 1.706 | |
| Na2S x 9 H2O | 0.30 | g | 0.3 | 1.248 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except bicarbonate, vitamins, glucose, cysteine and sulfide), then sparge medium with 100% CO2 gas for 30 - 45 min to make it anoxic. Add the bicarbonate and equilibrate the medium with the CO2 gas to pH 6.8. Distribute medium under CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Thereafter, add vitamins, glucose, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Vitamins are sterilized by filtration. Adjust pH of complete medium to 6.7 - 6.8, if necessary. | |||||
Media with this solution
| 712 | PSEUDOBUTYRIVIBRIO MEDIUM |