Main sol. 717
Internal Solution-ID: S1468
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
(NH4)H2PO4 | 2.88 | g | 2.846 | - | |
MgSO4 x 7 H2O | 0.10 | g | 0.099 | 0.401 | |
Ca(NO3)2 x 4 H2O | 0.05 | g | 0.049 | 0.209 | |
Modified Wolin's mineral solution | 10.00 | ml | - | - | |
Yeast extract | 0.10 | g | 0.099 | - | |
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 4.9e-4 | 0.002 | |
Na2CO3 | 1.50 | g | 1.482 | 13.985 | |
Na-L-lactate | 5.00 | g | 4.941 | - | |
Na2-fumarate | 3.20 | g | 3.162 | 19.758 | |
Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
Seven vitamins solution | 1.00 | ml | - | - | |
Na2S x 9 H2O | 0.10 | g | 0.099 | 0.411 | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve ingredients except carbonate, lactate, fumarate, vitamins and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic, then dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add lactate, fumarate, vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Stock solutions of fumarate and vitamins should be sterilized by filtration. The pH of the complete medium should be at 7.0 - 7.2. |
717 | DESULFITOBACTERIUM (PCE) MEDIUM |