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Main sol. 717

Internal Solution-ID: S1468

Equipment needed: Autoclave Hungate tubes Filter Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)H2PO4 2.88 g 2.846 -
MgSO4 x 7 H2O 0.10 g 0.099 0.401
Ca(NO3)2 x 4 H2O 0.05 g 0.049 0.209
Modified Wolin's mineral solution 10.00 ml - -
Yeast extract 0.10 g 0.099 -
Sodium resazurin
(0.1% w/v)
0.50 ml 4.9e-4 0.002
Na2CO3 1.50 g 1.482 13.985
Na-L-lactate 5.00 g 4.941 -
Na2-fumarate 3.20 g 3.162 19.758
Wolin's vitamin solution (10x) 1.00 ml - -
Seven vitamins solution 1.00 ml - -
Na2S x 9 H2O 0.10 g 0.099 0.411
Distilled water 1000.00 ml - -
1 Dissolve ingredients except carbonate, lactate, fumarate, vitamins and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic, then dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add lactate, fumarate, vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Stock solutions of fumarate and vitamins should be sterilized by filtration. The pH of the complete medium should be at 7.0 - 7.2.
717 DESULFITOBACTERIUM (PCE) MEDIUM