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Main sol. 719

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Internal Solution-ID: S1471

Equipment needed: Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.50 g 0.489 3.592
MgSO4 x 7 H2O 0.40 g 0.391 1.586
NaCl 0.40 g 0.391 6.691
NH4Cl 0.40 g 0.391 7.31
CaCl2 x 2 H2O 0.05 g 0.049 0.332
FeSO4 x 7 H2O solution (0.1% w/v) 2.00 ml - -
Trace element solution SL-10 1.00 ml - -
Yeast extract 1.00 g 0.978 -
Na-acetate 1.00 g 0.978 11.916
Na-formate 2.00 g 1.955 28.748
Fatty acid mixture 20.00 ml - -
Sodium resazurin
(0.1% w/v) 		0.50 ml 4.9e-4 0.002
NaHCO3 4.00 g 3.91 46.545
D-Glucose 5.00 g 4.888 27.129
L-Cysteine HCl x H2O 0.50 g 0.489 2.783
Na2S x 9 H2O 0.50 g 0.489 2.035
Distilled water 1000.00 ml - -
1 Dissolve ingredients except bicarbonate, glucose, cysteine and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add and dissolve bicarbonate, then dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add glucose, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Prior to use check pH of complete medium and adjust to 6.8 - 7.0, if necessary.
719 ANAEROFILUM MEDIUM