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Main sol. 1004

Internal Solution-ID: S15

Equipment needed: Autoclave Filter Hungate tubes
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.14 g 0.138 1.017
2 0.20 g 0.198 0.972
3 0.15 g 0.148 1.008
4 0.54 g 0.534 9.975
5 Trace element solution SL-11 1.00 ml - -
6 Selenite-tungstate solution 1.00 ml - -
7
(0.1% w/v)
0.50 ml 4.9e-4 0.002
8 2.50 g 2.47 29.407
10 Vitamin solution 10.00 ml - -
12 0.25 g 0.247 1.407
13 0.25 g 0.247 1.029
14 1000.00 ml 986.371 54751.8
Instructions:
  1. Dissolve ingredients (except bicarbonate, yeast extract, vitamins, glucose and reducing agents), then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add solid bicarbonate and adjust pH to 7.0. Dispense medium under 80% N2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add yeast extract, vitamins (sterilized by filtration), glucose, sulfide and cysteine from sterile anoxic stock solutions prepared under 100% N2 gas. Adjust pH of complete medium to 7.0, if necessary.
1004 ANAEROLINEA MEDIUM