Main sol. 733
Internal Solution-ID: S1503
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na-acetate | 20.00 | mM | 1.641 | 20 | |
| HEPES | 8.00 | g | 7.992 | 33.538 | |
| K2HPO4 | 1.00 | g | 0.999 | 5.736 | |
| NH4Cl | 0.50 | g | 0.5 | 9.338 | |
| Yeast extract | 1.00 | g | 0.999 | - | |
| Peptone | 0.50 | g | 0.5 | - | |
| NaCl | 15.00 | g | 14.985 | 256.417 | |
| MgSO4 x 7 H2O | 1.00 | g | 0.999 | 4.053 | |
| CaCl2 x 2 H2O | 0.05 | g | 0.05 | 0.34 | |
| Modified Wolin's mineral solution | 1.00 | ml | - | - | |
| Biotin | 0.10 | g | 0.1 | 0.409 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Prepare the HEPES, NaCl, K2HPO4 and NH4Cl as a basal salt solution, adjusting the pH to 7.5-7.8 with NaOH before sterilisation. All other components are added from sterile stock solutions. | |||||
Media with this solution
| 733 | SULFITOBACTER PONTIACUS MEDIUM |