Main sol. 742
Internal Solution-ID: S1520
Equipment needed: Autoclave
Hungate tubes
Filter
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 10.0 | g | 9.891 | 169.254 | |
| Na2SO4 | 3.0 | g | 2.967 | 20.891 | |
| Na2HPO4 | 0.2 | g | 0.198 | 1.394 | |
| KCl | 0.2 | g | 0.198 | 2.654 | |
| NH4Cl | 1.0 | g | 0.989 | 18.492 | |
| Modified Wolin's mineral solution | 10.0 | ml | - | - | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 4.9e-4 | 0.002 | |
| NaHCO3 | 15.0 | g | 14.837 | 176.614 | |
| Na2CO3 | 10.0 | g | 9.891 | 93.324 | |
| Yeast extract | 1.5 | g | 1.484 | - | |
| Na-formate | 5.0 | g | 4.946 | 72.722 | |
| Wolin's vitamin solution (10x) | 1.0 | ml | - | - | |
| Na2S x 9 H2O | 1.0 | g | 0.989 | 4.118 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve ingredients except bicarbonate, carbonate, yeast extract, vitamins, formate, and sulfide. Sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic, then add bicarbonate and carbonate and adjust pH to 9.5. Dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add yeast extract, vitamins, formate and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Vitamins should be sterilized by filtration. The pH of the complete medium should be at 9.5 - 9.7. | |||||
Media with this solution
| 742 | DESULFONATRONOVIBRIO MEDIUM |