Main sol. 771
Internal Solution-ID: S1575
Equipment needed: Autoclave
Hungate tubes
Filter
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| K2HPO4 | 225.00 | mg | 0.224 | 1.288 | |
| KH2PO4 | 225.00 | mg | 0.224 | 1.648 | |
| NaCl | 460.00 | mg | 0.459 | 7.848 | |
| (NH4)2SO4 | 225.00 | mg | 0.224 | 1.698 | |
| MgSO4 x 7 H2O | 117.00 | mg | 0.117 | 0.473 | |
| Yeast extract | 1.00 | g | 0.997 | - | |
| Trace element solution SL-10 | 1.00 | ml | - | - | |
| Selenite-tungstate solution | 1.00 | ml | - | - | |
| Na2CO3 | 1.50 | g | 1.496 | 14.11 | |
| Na-DL-lactate | 2.25 | g | 2.243 | 20.019 | |
| NaNO3 | 1.70 | g | 1.695 | 19.941 | |
| Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
| L-Cysteine HCl x H2O | 0.10 | g | 0.1 | 0.568 | |
| Na2S x 9 H2O | 0.10 | g | 0.1 | 0.415 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients except carbonate, lactate, nitrate, vitamins, cysteine and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic, then dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add lactate, nitrate, vitamins, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Vitamins should be sterilized by filtration. The pH of the complete medium should be 7.2 - 7.4. | |||||
Media with this solution
| 771 | SULFUROSPIRILLUM BARNESII MEDIUM |