Main sol. 772
Internal Solution-ID: S1576
Equipment needed: Autoclave
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| (NH4)2SO4 | 3.0 | g | 3 | 22.703 | |
| KH2PO4 | 1.4 | g | 1.4 | 10.288 | |
| Na2HPO4 | 3.0 | g | 3 | 21.133 | |
| MgSO4 x 7 H2O | 0.2 | g | 0.2 | 0.811 | |
| Fe(III) citrate | 30.0 | mg | 0.03 | 0.122 | |
| CaCl2 x 2 H2O | 30.0 | mg | 0.03 | 0.204 | |
| MnCl2 x 4 H2O | 5.0 | mg | 0.005 | 0.025 | |
| ZnSO4 x 7 H2O | 5.0 | mg | 0.005 | 0.017 | |
| CuSO4 x 2 H2O | 0.5 | mg | 5.0e-4 | 0.003 | |
| Thiamine-HCl x 2 H2O | 0.4 | mg | 4.0e-4 | 0.001 | |
| Distilled water | 1000.0 | ml | - | - | |
|
Methanol
(filter-sterilized) |
10.0 | ml | 7.92 | 247.176 | |
| 1 Thiamine hydrochloride may be replaced by 2 g/l yeast extract. The pH of the medium is adjusted to pH 9.0 with filter sterilised 10% Na2CO3. 10 ml sterile methanol (filter sterilised, or autoclaved in crimp top sealed bottles) is added to the sterilised medium. | |||||
Media with this solution
| 772 | PARACOCCUS ALCALIPHILUS MEDIUM |