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Main sol. 775

Internal Solution-ID: S1579

Equipment needed: Autoclave Wheaton tubes Hungate tubes
Compound Amount Unit Conc. [g/L] Conc. [mM]
Trypticase 5.0 g 5 -
Tryptone 5.0 g 5 70.343
Yeast extract 5.0 g 5 -
KH2PO4 5.0 g 5 36.742
Na-acetate 5.0 g 5 60.951
(NH4)2 citrate 2.0 g 2 -
MgSO4 x 7 H2O 0.5 g 0.5 2.029
MnSO4 x H2O 0.2 g 0.2 1.183
Tween 80 1.0 ml - -
Cheese whey 1000.0 ml - -
1 Adjust pH to 5.5 and distribute into screw-capped Wheaton tubes or Hungate tubes.
775 R - CW MEDIUM