Main sol. 779
Internal Solution-ID: S1584
Equipment needed: Autoclave
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Lab-Lemco beef extract
(Oxoid) |
2.4 | g | 2.4 | - | |
|
Proteose peptone no. 3
(Difco) |
10.0 | g | 10 | - | |
|
Yeast extract
(Difco, Bacto) |
5.0 | g | 5 | - | |
| Na2HPO4 | 4.0 | g | 4 | 28.177 | |
| Glucose | 1.5 | g | 1.5 | 8.326 | |
|
Starch
(soluble) |
0.5 | g | 0.5 | - | |
| L-Cysteine HCl x H2O | 0.5 | g | 0.5 | 2.847 | |
| Agar , if necessary | 15.0 | g | 15 | 44.599 | |
| Horse blood | 50.0 | ml | - | - | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Adjust pH to 7.6 - 7.8. | |||||
| 2 Add the horse blood to the heat sterilized medium after being cooled to 50°C. Incubate under air plus 5% CO2 or anaerobically under 95% N2 plus 5% CO2. | |||||
Media with this solution
| 779 | CAPNOCYTOPHAGA II MEDIUM |