Main sol. 784
Internal Solution-ID: S1589
Equipment needed: Gassing station
Autoclave
Hungate tubes
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.2 | g | 0.199 | 1.459 | |
| MgCl2 x 6 H2O | 0.1 | g | 0.099 | 0.488 | |
| NH4Cl | 1.0 | g | 0.993 | 18.565 | |
| KCl | 0.2 | g | 0.199 | 2.664 | |
| NaCl | 16.0 | g | 15.889 | 271.882 | |
| Trace element solution SL-11 | 1.0 | ml | - | - | |
| Yeast extract | 0.2 | g | 0.199 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | 0.002 | |
| Na2CO3 | 68.0 | g | 67.527 | 637.122 | |
| NaHCO3 | 38.0 | g | 37.736 | 449.199 | |
| Ethanol absolute | 5.0 | ml | - | - | |
| Wolin's vitamin solution (10x) | 1.0 | ml | - | - | |
| Na2S x 9 H2O | 1.0 | g | 0.993 | 4.135 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve ingredients except carbonate, bicarbonate, ethanol, vitamins and sulfide. Sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add and dissolve carbonates and sulfide while gassing the head space only. Dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Before use add ethanol and vitamins from sterile anoxic stock solution prepared under 100% N2 gas. Vitamins are sterilized by filtration. Adjust pH of the complete medium to 9.5 - 10.0, if necessary. | |||||
Media with this solution
| 784 | NATRONIELLA MEDIUM |