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Main sol. 1005

Internal Solution-ID: S16

Equipment needed: Autoclave Hungate tubes Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.78 g 0.765 4.39
2 0.75 g 0.735 5.403
3 0.04 g 0.039 0.117
4 0.01 g 0.01 0.035
5 0.25 g 0.245 0.994
6 0.03 g 0.029 0.2
7 0.20 g 0.196 3.355
8 0.50 g 0.49 9.164
9 2.80 g 2.745 19.326
10 0.15 g 0.147 1.793
11 Trace element solution 10.00 ml - -
12 Vitamin solution 10.00 ml - -
13 0.25 g 0.245 1.395
14 1000.00 ml 978.634 54322.4
Instructions:
  1. Dissolve ingredients (except vitamins and cysteine), adjust pH to 5.5 with 10 N H2SO4 and sparge medium with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials (e.g., 20 ml of medium in 50 ml bottles) and autoclave. Prior to inoculation add vitamins (sterilized by filtration) and cysteine from sterile anoxic stock solutions prepared under 100% N2 gas. Adjust pH of complete medium to 5.5 - 6.0, if necessary. After inoculation pressurize vials to 0.5 bar overpressure with sterile 80% H2 and 20% CO2 gas mixture.
1005 THERMODESULFOBIUM MEDIUM