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Solution A

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Internal Solution-ID: S1607

Equipment needed: Gassing station Serum bottle Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 20.00 g 20.222 346.038
MgSO4 x 7 H2O 3.50 g 3.539 14.358
MgCl2 x 6 H2O 2.75 g 2.781 13.677
CaCl2 x 2 H2O 0.75 g 0.758 5.158
KCl 0.33 g 0.334 4.476
NaBr 0.05 g 0.051 0.491
H3BO3 15.00 mg 0.015 0.245
SrCl2 x 6 H2O
(0.1% w/v) 		7.00 ml 0.007 0.027
KI
(0.01% w/v) 		0.50 ml 5.1e-5 1.9e-4
Wolfe's mineral elixir 1.00 ml - -
Sodium resazurin
(0.1% w/v) 		0.50 ml 5.1e-4 0.002
Distilled water 980.00 ml - -
792a THERMOVIBRIO AMMONIFICANS MEDIUM