Main sol. 797
Internal Solution-ID: S1626
Equipment needed: Hungate tubes
Filter
Gassing station
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Ground beef
(fat free) |
500.0 | g | 500 | - | |
| Distilled water | 1000.0 | ml | - | - | |
|
NaOH
(1 N) |
25.0 | ml | - | - | |
| 1 Mix meat, water and NaOH, and bring to a boil with stirring. Cool to room temperature, skim fat off surface, and filter, retaining both meat particles and filtrate. To the filtrate add sufficient distilled water to restore volume to 1000 ml. To this filtrate add: | |||||
|
Trypticase
(BBL 11921) |
30.0 | g | 30 | - | |
| Yeast extract | 5.0 | g | 5 | - | |
| K2HPO4 | 5.0 | g | 5 | 28.707 | |
|
Resazurin
(0.025%) |
4.0 | ml | 1.0e-3 | 0.004 | |
| Agar , if required | 20.0 | g | 20 | 59.465 | |
| 2 Boil and cool medium under 80% N2, 10% H2, 10% CO2, and add: | |||||
| L-Cysteine HCl x H2O | 0.5 | g | 0.5 | 2.847 | |
| Haemin solution | 10.0 | ml | - | - | |
| Vitamin K1 solution | 0.2 | ml | - | - | |
| 3 Adjust medium for final pH 7.0. Anaerobically dispense 7 ml into tubes containing meat particles (use 1 part meat to 5 parts fluid) under the same gas phase. | |||||
Media with this solution
| 797 | MODIFIED CHOPPED MEAT MEDIUM |