Main sol. 798
Internal Solution-ID: S1629
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Na2CO3 | 8.0 | g | 8 | 75.48 | |
KCl | 0.2 | g | 0.2 | 2.683 | |
NaCl | 10.0 | g | 10 | 171.116 | |
NH4Cl | 0.5 | g | 0.5 | 9.348 | |
K2HPO4 | 0.2 | g | 0.2 | 1.148 | |
Yeast extract | 4.0 | g | 4 | - | |
Trace element solution SL-10 | 1.0 | ml | - | - | |
Wolin's vitamin solution | 1.0 | ml | - | - | |
Na2S x 9 H2O | 0.5 | g | 0.5 | 2.082 | |
Distilled water | 1000.0 | ml | - | - | |
1 pH 9.0 | |||||
2 Prepare the medium anaerobically without the vitamins. Boil the medium, cool under N2 and dispense under N2 and autoclave the medium. Add the vitamin solution to the cooled, autoclaved medium. |
798 | TINDALLIA MEDIUM |