Main sol. 95
Internal Solution-ID: S164
Equipment needed: Autoclave
Gassing station
Hungate tubes
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Solution A | 951 | ml | - | - | |
| Solution B | 3 | ml | - | - | |
| Solution C | 40 | ml | - | - | |
| Solution D | 10 | ml | - | - | |
| 1 Sparge solution A with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense solution under same gas atmosphere into anoxic Hungate-type tubes or serum vials and sterilize by autoclaving. Solutions B and D are autoclaved separately under 100% N2 gas atmosphere. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. The medium is completed before use by adding appropriate amounts of solutions B, C and D to solution A. Adjust pH of complete medium to 7.2, if necessary. | |||||
Media with this solution
| 95 | DESULFUROMONAS ACETOXIDANS MEDIUM |