Main sol. 812
Internal Solution-ID: S1649
Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| (NH4)2SO4 | 3.0 | g | 3 | 22.703 | |
| KCl | 0.1 | g | 0.1 | 1.341 | |
| KH2PO4 | 0.5 | g | 0.5 | 3.674 | |
| MgSO4 x 7 H2O | 0.5 | g | 0.5 | 2.029 | |
| Ca(NO3)2 x 4 H2O | 0.1 | g | 0.1 | 0.423 | |
| Yeast extract | 0.1 | g | 0.1 | - | |
| Distilled water | 1000.0 | ml | - | - | |
|
Glutathione
(10% w/v) |
10.0 | ml | 1 | 3.254 | |
| 1 Adjust pH to 2.25 prior to autoclaving. Before use add per liter 10 ml of a filter sterilized 10% (w/v) glutathione solution. | |||||
Media with this solution
| 812 | SULFOBACILLUS DISULFIDOOXIDANS MEDIUM |