Main sol. 817
Internal Solution-ID: S1665
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
MgCl2 x 6 H2O | 2.2 | g | 2.167 | 10.661 | |
NaCl | 0.9 | g | 0.887 | 15.173 | |
KCl | 17.0 | mg | 0.017 | 0.225 | |
NH4Cl | 12.0 | mg | 0.012 | 0.221 | |
CaCl2 x 2 H2O
(0.1% w/v) |
7.0 | ml | 0.007 | 0.047 | |
K2HPO4 x 3 H2O
(0.1% w/v) |
7.0 | ml | 0.007 | 0.04 | |
FeCl3
(0.01% w/v in 0.2 N HCl) |
0.5 | ml | 4.9e-5 | 3.0e-4 | |
Yeast extract
(BD Bacto) |
1.0 | g | 0.985 | - | |
Peptone
(BD Bacto) |
1.0 | g | 0.985 | - | |
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 4.9e-4 | 0.002 | |
Na2CO3 | 0.5 | g | 0.493 | 4.648 | |
Wolin's vitamin solution (10x) | 1.0 | ml | - | - | |
Na2S x 9 H2O | 0.5 | g | 0.493 | 2.051 | |
Distilled water | 1000.0 | ml | - | - | |
1 Dissolve ingredients except carbonate, vitamins and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic, then dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials to 30% of their volume and autoclave. Add vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Vitamins should be sterilized by filtration. The pH of the complete medium should be adjusted to 6.5. | |||||
2 After inoculation pressurize tubes or bottles (heavy walled) to 2 bar overpressure with sterile 80% N2 and 20% CO2 gas mixture. |
817 | THERMOSPHAERA MEDIUM |