Main sol. 828
Internal Solution-ID: S1677
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Potassium phosphate buffer , 1M, pH 7 | 27.50 | ml | 64.35 | - | |
MgSO4 x 7 H2O | 0.05 | g | 0.05 | 0.203 | |
CaCl2 x 2 H2O | 14.00 | mg | 0.014 | 0.095 | |
Na-lactate | 0.56 | g | 0.56 | 4.997 | |
SL-9 | 0.50 | ml | - | - | |
Seven vitamins solution | 0.50 | ml | - | - | |
Agar , if necessary | 15.00 | g | 15 | 44.599 | |
Distilled water | 972.50 | ml | - | - | |
1 Adjust the pH to 7.0 - 7.2. | |||||
2 Filter sterilize the vitamin solution and add to the autoclaved medium. |
828 | NEVSKIA MEDIUM |