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Main sol. 830d

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Internal Solution-ID: S1690

Equipment needed: Autoclave Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 0.50 g 0.5 -
Proteose peptone
(BD DIFCO no. 3) 		0.50 g 0.5 -
Casamino acids 0.50 g 0.5 -
Glucose 0.50 g 0.5 2.775
Starch , soluble 0.50 g 0.5 -
Na-pyruvate 0.30 g 0.3 2.726
K2HPO4 0.30 g 0.3 1.722
MgSO4 x 7 H2O 0.05 g 0.05 0.203
Distilled water 1000.00 ml - -
1 Dissolve ingredients, then sparge solution with 80% N2 and 20% CO2 gas mixture for at least 30 - 45 min to remove dissolved oxygen and to saturate the solution with CO2. Dispense solution in vials suitable for anaerobic cultures (e.g. Balch-type tubes or serum vials) to 75% volume under air atmosphere. Close vials with butyl rubber septa to prevent free exchange of oxygen with the external atmosphere and autoclave. Adjust pH of the autoclaved medium to 5.0 - 5.5 using a sterile stock solution of sodium carbonate (5% w/v), prepared under 80% N2 and 20% CO2 gas mixture.
830d R2A MEDIUM (MICROAEROBIC)