Main sol. 834
Internal Solution-ID: S1701
Equipment needed: Hungate tubes
Filter
Autoclave
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Meat peptone
(BD Bacto) |
5.00 | g | 4.985 | - | |
|
Tryptone
(BD Bacto) |
5.00 | g | 4.985 | - | |
| Yeast extract | 10.00 | g | 9.97 | - | |
| KH2PO4 | 0.30 | g | 0.299 | 2.198 | |
| NaCl | 0.60 | g | 0.598 | 10.236 | |
| MgCl2 x 6 H2O | 0.10 | g | 0.1 | 0.49 | |
| CaCl2 x 2 H2O | 0.08 | g | 0.08 | 0.543 | |
| Trace element solution SL-10 | 1.00 | ml | - | - | |
| Selenite-tungstate solution | 1.00 | ml | - | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | 0.002 | |
| Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
| L-Cysteine HCl x H2O | 0.25 | g | 0.249 | 1.419 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except vitamins and cysteine), boil medium for 1 min, then cool to room temperature under 100% N2 gas atmosphere. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Before inoculation, add vitamins (sterilized by filtration) and cysteine from sterile anoxic stock solution prepared under 100% N2 gas. Adjust pH of complete medium to 7.0, if necessary. | |||||
Media with this solution
| 834 | COPROTHERMOBACTER PLATENSIS MEDIUM |