Main sol. 836

Equipment needed: Hungate tubes Filter Autoclave Gassing station

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
KH₂PO₄	0.27	g	0.27	1.984
K₂HPO₄	0.35	g	0.35	2.009
NH₄Cl	0.53	g	0.53	9.908
MgCl₂ x 6 H₂O	0.10	g	0.1	0.492
FeCl₂ x 4 H₂O (0.1% w/v in 0.2 N HCl)	2.00	ml	0.002	0.01
CaCl₂ x 2 H₂O	0.07	g	0.07	0.476
Trace element solution SL-10	1.00	ml	-	-
Yeast extract	1.00	g	1	-
Na-pyruvate	6.05	g	6.05	54.978
Sodium resazurin (0.1% w/v)	0.50	ml	5.0e-4	-
Na₂CO₃	0.50	g	0.5	4.718
Na₂SO₃	0.63	g	0.63	4.998
Na₂S x 9 H₂O	0.05	g	0.05	0.208
Distilled water	1000.00	ml	-	-
1 Dissolve ingredients (except carbonate, sulfite and sulfide), adjust pH of solution to 7.0 and sparge with 100% N₂ gas for 30 - 45 min to make it anoxic. Dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Prior to use add sulfite and sulfide from sterile anoxic stock solutions prepared under 100% N₂ gas and carbonate from a sterile anoxic stock solution prepared under 80% N₂ and 20% CO₂ gas atmosphere. The stock solution of sulfite should by freshly prepared and sterilized by filtration. Prior to use check pH of complete medium and adjust to 7.5, if necessary.

836	DESULFITOBACTERIUM PCP-1 MEDIUM