Back to all solutions

Main sol. 837

Internal Solution-ID: S1710

Equipment needed: Gassing station Hungate tubes Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 870 ml - -
Solution B 50 ml - -
Solution C 20 ml - -
Solution D 10 ml - -
Solution E 50 ml - -
1 Sparge solution A with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic, distribute under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Solution B is autoclaved separately under 100% N2 gas atmosphere. Solution C is prepared under 80% N2 and 20% CO2 gas atmosphere. Solutions D and E are prepared under 100% N2 gas atmosphere and sterilized by filtration. To complete the medium appropriate amounts of solutions B to E are added to the sterile solution A in the sequence as indicated. Adjust pH of complete medium to 6.9, if necessary. Prior to inoculation medium is reduced by addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized).
2 Note: For transfers use 5 - 10% (v/v) inoculum.