Main sol. 845
Internal Solution-ID: S1732
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Peptone
(BD Bacto) |
5 | g | 4.985 | - | |
Disodium succinate | 1 | g | 0.997 | 6.152 | |
(NH4)2SO4 | 1 | g | 0.997 | 7.545 | |
MgSO4 x 7 H2O | 1 | g | 0.997 | 4.045 | |
FeCl3 x 6 H2O | 2 | mg | 0.002 | 0.007 | |
MnSO4 x H2O | 2 | mg | 0.002 | 0.012 | |
Agar
(BD Bacto) |
1 | g | 0.997 | - | |
Trace element solution SL-11 | 1 | ml | - | - | |
Seven vitamins solution | 1 | ml | - | - | |
Catalase
(2000 U/ml) |
1 | ml | - | - | |
Distilled water | 1000 | ml | - | - | |
1 Dissolve ingredients except trace elements, vitamins and catalase, adjust pH to 7.2 - 7.4, bring medium to the boil and cool to room temperature under 100% N2 gas atmosphere. Dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. To complete the medium add trace elements, vitamins and catalase from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere. Vitamins and catalase should be sterilized by filtration. After inoculation, inject sterile air into the culture vessel up to about 20% of the total gas volume (i.e., 4 parts N2 and 1 part air). |
845 | SPIRILLUM WINOGRADSKYI MEDIUM |