Main sol. 847
Internal Solution-ID: S1735
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
(NH4)2SO4 | 0.30 | g | 0.3 | 2.27 | |
MgSO4 x 7 H2O | 0.30 | g | 0.3 | 1.217 | |
CaCl2 x 2 H2O | 0.15 | g | 0.15 | 1.02 | |
Yeast extract
(OXOID) |
0.20 | g | 0.2 | - | |
Agar
(BD Bacto) |
1.00 | g | 1 | 2.973 | |
Seven vitamins solution | 1.00 | ml | - | - | |
Trace element solution SL-11 | 1.00 | ml | - | - | |
Na2S2O3 x 5 H2O | 1.00 | g | 1 | 4.029 | |
Disodium succinate | 0.50 | g | 0.5 | 3.085 | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve ingredients except vitamins, trace elements, thiosulfate and succinate. Adjust pH to 7.2 - 7.4 and bring medium to the boil, distribute into culture vessels and autoclave. To complete the medium add vitamins, trace elements, thiosulfate and succinate from stock solutions sterilized by filtration. |
847 | MACROMONAS MEDIUM |