Main sol. 859
Internal Solution-ID: S1758
Equipment needed: Autoclave
Wheaton tubes
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Tryptone | 10.00 | g | 10 | 140.687 | |
| Meat extract | 3.00 | g | 3 | - | |
| Yeast extract | 5.00 | g | 5 | - | |
| Glucose | 4.00 | g | 4 | 22.203 | |
| Maltose | 4.00 | g | 4 | 11.686 | |
| Na-acetate x 3 H2O | 5.00 | g | 5 | - | |
| (NH4)2 citrate | 2.00 | g | 2 | - | |
| K2HPO4 x 3 H2O | 2.60 | g | 2.6 | 14.927 | |
| MgSO4 x 7 H2O | 0.10 | g | 0.1 | 0.406 | |
| MnSO4 x 4 H2O | 0.05 | g | 0.05 | 0.224 | |
| L-Cysteine HCl x H2O | 0.50 | g | 0.5 | 2.847 | |
| Tween 80 | 1.00 | ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Adjust pH to 6.3 and fill up into screw-capped Wheaton tubes. | |||||
Media with this solution
| 859 | LACTOBACILLUS MEDIUM IV |