Main sol. 104c
Internal Solution-ID: S179
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Trypticase peptone
(BD BBL) |
5.0 | g | 5 | - | |
Meat peptone
(pepsin-digested) |
5.0 | g | 5 | - | |
Yeast extract | 10.0 | g | 10 | - | |
Salt solution | 40.0 | ml | - | - | |
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | 0.002 | |
L-Cysteine HCl x H2O | 0.5 | g | 0.5 | 2.847 | |
Na2CO3 | 1.0 | g | 1 | 9.435 | |
D-Glucose | 5.0 | g | 5 | 27.753 | |
Distilled water | 960.0 | ml | - | - | |
1 Dissolve ingredients (except cysteine, carbonate and glucose) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add cysteine, then dispense under 80% N2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add glucose from a sterile anoxic stock solution prepared under 100% N2 gas atmosphere and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Adjust pH of complete medium to 7.0, if necessary. |
104c | PY + X MEDIUM (N2/CO2) |