Main sol. 869
Internal Solution-ID: S1797
Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Trypticase soy broth
(BD BBL) |
5.5 | g | 5.5 | - | |
|
Resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | 0.002 | |
| D-Glucose | 2.0 | g | 2 | 11.101 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve dehydrated TSB medium and resazurin, bring to the boil and cool to room temperature while sparging with 100% N2 gas. Adjust pH to 5.5, dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add glucose from a separately sterilized anoxic stock solution. | |||||
Media with this solution
| 869 | ANAEROBIC TRYPTIC SOY BROTH MEDIUM |