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Na-crotonate solution (1 M)

Internal Solution-ID: S1799

This solution is used in 4 mediaand was first defined in medium 870.

Compound Amount Unit Conc. [g/L] Conc. [mM]
Crotonic acid
(ALDRICH 113018)
86 g 86 998.955
NaOH
(10 N)
100 ml - -
Distilled water 900 ml - -
1 Dissolve crotonic acid in 800 ml distilled water, add around 100 ml of 10 N NaOH and adjust pH to around 7. Then add water to reach a volume of 1000 ml. Sterilize by filtration under 100% N2 gas atmosphere.
870 SYNTROPHOTHERMUS MEDIUM
1030 SMITHELLA MEDIUM
1086 DESULFATIRHABDIUM MEDIUM
1662 ALKALIPHILUS HYDROTHERMALIS MEDIUM