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Main sol. 872

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Internal Solution-ID: S1802

Equipment needed: Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.30 g 0.3 5.603
K2HPO4 0.20 g 0.2 1.147
MgCl2 x 6 H2O 0.40 g 0.4 1.966
CaCl2 x 2 H2O 0.10 g 0.1 0.68
KCl 0.50 g 0.5 6.7
NaCl 1.00 g 0.999 17.095
Yeast extract
(BD Bacto) 		5.00 g 4.995 -
Trypticase peptone
(BD BBL) 		5.00 g 4.995 -
Trace element solution SL-10 1.00 ml - -
Sodium resazurin
(0.1% w/v) 		0.50 ml 5.0e-4 0.002
Na2CO3 2.00 g 1.998 18.851
trans-Cinnamic acid 0.75 g 0.749 5.057
L-Cysteine HCl x H2O 0.50 g 0.5 2.844
Na2S x 9 H2O 0.50 g 0.5 2.08
Distilled water 1000.00 ml - -
1 Dissolve all ingredients except carbonate, cinnamic acid, sulfide and cysteine, then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add cinnamic acid, sulfide and cysteine from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Adjust pH of complete medium to 7.2 - 7.5, if necessary.
872 PAPILLIBACTER MEDIUM