MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder Unit converter Compare media Compare solutions Prediction SOON
DSMZ
Back to all solutions

Main sol. 879

Download this solution as

CSV JSON PDF

Internal Solution-ID: S1820

Equipment needed: Autoclave Hungate tubes Filter Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 1.00 g 0.989 18.492
KCl 0.10 g 0.099 1.327
MgSO4 x 7 H2O 0.20 g 0.198 0.803
NaCl 0.80 g 0.791 13.54
KH2PO4 0.10 g 0.099 0.727
CaCl2 x 2 H2O 0.02 g 0.02 0.135
Modified Wolin's mineral solution 10.00 ml - -
Sodium resazurin
(0.1% w/v) 		0.50 ml 4.9e-4 0.002
Yeast extract 1.00 g 0.989 -
NaHCO3 1.00 g 0.989 11.774
D-Fructose 5.00 g 4.946 27.451
Wolin's vitamin solution (10x) 1.00 ml - -
L-Cysteine HCl x H2O 0.30 g 0.297 1.689
Na2S x 9 H2O 0.30 g 0.297 1.235
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except bicarbonate, yeast extract, fructose, vitamins, and reducing agents), sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic and adjust pH to 5.0. Dispense medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave at 121°C for 15 min. Add yeast extract, fructose, vitamins (sterilized by filtration), cysteine, and sulfide from sterile stock solutions prepared under a 100% N2 gas atmosphere and bicarbonate from a sterile stock solution prepared under an 80% N2 and 20% CO2 gas atmosphere. Adjust the pH of the complete medium to 5.9!
879 CLOSTRIDIUM LJUNGDAHLII MEDIUM