Main sol. 880
Internal Solution-ID: S1821
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
KH2PO4 | 0.30 | g | 0.298 | 2.187 | |
NaCl | 0.60 | g | 0.595 | 10.186 | |
MgCl2 x 6 H2O | 0.10 | g | 0.099 | 0.488 | |
CaCl2 x 2 H2O | 0.08 | g | 0.079 | 0.54 | |
NH4Cl | 1.00 | g | 0.992 | 18.547 | |
Trace element solution SL-11 | 1.00 | ml | - | - | |
Selenite-tungstate solution | 1.00 | ml | - | - | |
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | 0.002 | |
KHCO3 | 3.50 | g | 3.472 | 34.682 | |
L-Cysteine HCl x H2O | 0.60 | g | 0.595 | 3.389 | |
Methanol
(50% v/v) |
6.00 | ml | 4.714 | 147.128 | |
Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
Na2S x 9 H2O | 0.30 | g | 0.298 | 1.239 | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve ingredients (except bicarbonate, cysteine, methanol, vitamins and sulfide), sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic, then add solid bicarbonate and cysteine and equilibrate the medium with the gas mixture to reach a pH of 7.0. Dispense medium under 80% N2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. To the autoclaved medium add methanol (50% v/v solution), vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Vitamins should be sterilized by filtration. Adjust pH of complete medium to 7.0 - 7.2, if necessary. |
880 | THERMOACETOGENIUM MEDIUM |