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Main sol. 881

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Internal Solution-ID: S1822

Equipment needed: Autoclave Hungate tubes Filter Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2SO4 0.02 g 0.02 0.14
KH2PO4 1.00 g 0.997 7.326
NH4Cl 0.25 g 0.249 4.66
NaCl 20.00 g 19.94 341.208
MgCl2 x 6 H2O 3.00 g 2.991 14.712
KCl 0.50 g 0.499 6.687
CaCl2 x 2 H2O 0.15 g 0.15 1.017
Na-acetate 1.64 g 1.635 19.932
Trace element solution SL-10 1.00 ml - -
Selenite-tungstate solution 1.00 ml - -
Sodium resazurin
(0.1% w/v) 		0.50 ml 5.0e-4 0.002
Na2CO3 1.00 g 0.997 9.407
Na2-fumarate 6.40 g 6.381 39.871
Seven vitamins solution 1.00 ml - -
Na2S x 9 H2O 0.50 g 0.499 2.076
Distilled water 1000.00 ml - -
1 Dissolve all ingredients except carbonate, fumarate, vitamins and sulfide, then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add fumarate, vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Vitamins should be sterilized by filtration. Adjust pH of complete medium to 7.0 - 7.2, if necessary.
881 DENITROVIBRIO MEDIUM