Main sol. 882
Internal Solution-ID: S1823
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Solution A | 950 | ml | - | - | |
| Solution B | 50 | ml | - | - | |
| Solution C | 1 | ml | - | - | |
| 1 Sterilize solution A and solution C by autoclaving at 121°C for 20 min and solution B by autoclaving at 112°C for 30 min. Prior to use mix solutions A and B and add trace elements solution. The final pH of the medium should be 2.3. | |||||
| 2 Note: At the beginning of growth incubate statically without shaking. | |||||
Media with this solution
| 882 | LEPTOSPIRILLUM (HH) MEDIUM |