Main sol. 895
Internal Solution-ID: S1845
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
(NH4)2SO4 | 1.00 | g | 0.987 | 7.471 | |
NH4Cl | 0.40 | g | 0.395 | 7.382 | |
Na2S2O3 x 5 H2O | 0.10 | g | 0.099 | 0.398 | |
K2HPO4 | 0.50 | g | 0.494 | 2.834 | |
MgSO4 x 7 H2O | 0.10 | g | 0.099 | 0.401 | |
CaCl2 x 2 H2O | 0.05 | g | 0.049 | 0.336 | |
NaCl | 10.00 | g | 9.872 | 168.92 | |
FeSO4 x 7 H2O
(0.1% w/v in 0.1 N H2SO4) |
2.00 | ml | 0.002 | 0.007 | |
Modified Wolin's mineral solution | 10.00 | ml | - | - | |
Yeast extract
(BD Bacto) |
0.25 | g | 0.247 | - | |
Tryptone
(BD Bacto) |
0.25 | g | 0.247 | - | |
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 4.9e-4 | 0.002 | |
L-Cysteine HCl x H2O | 0.50 | g | 0.494 | 2.81 | |
Na2CO3 | 3.00 | g | 2.962 | 27.942 | |
Starch
(soluble) |
2.00 | g | 1.974 | - | |
Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve all ingredients except cysteine, carbonate, starch and vitamins. Sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic, add cysteine and adjust pH to 7.5, then dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add starch and vitamins from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Vitamins should be sterilized by filtration. Adjust pH of complete medium to 9.3 - 9.5, if necessary. |
895 | ANAEROBRANCA GOTTSCHALKII MEDIUM |