Main sol. 901a
Internal Solution-ID: S1854
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NH4Cl | 0.33 | g | 0.324 | 6.066 | |
KCl | 0.33 | g | 0.324 | 4.353 | |
KH2PO4 | 0.33 | g | 0.324 | 2.384 | |
MgCl2 x 6 H2O | 0.33 | g | 0.324 | 1.596 | |
CaCl2 x 2 H2O | 0.33 | g | 0.324 | 2.207 | |
Na2SO4 | 2.80 | g | 2.753 | 19.383 | |
Trace element solution SL-10 | 1.00 | ml | - | - | |
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 4.9e-4 | 0.002 | |
Yeast extract | 3.00 | g | 2.95 | - | |
Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
Neutralized sulfide solution 3% (w/v) | 15.00 | ml | - | - | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve ingredients except yeast extract, vitamins and sulfide. Adjust pH to 4.5 with H2SO4 and sparge medium with 100% CO2 gas for 30 - 45 min to make it anoxic. Dispense medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add yeast extract, vitamins (sterilized by filtration), and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. The sulfide stock solution (3% w/v) should be neutralized before use (see medium 28). The pH of the complete medium should be at 4.5. |
901a | THERMODESULFOBIUM ACIDIPHILUM MEDIUM |