Solution A
Internal Solution-ID: S1869
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Brain heart infusion broth | 12.50 | g | 12.5 | - | |
Trypticase
(BBL 11921) |
10.00 | g | 10 | - | |
Yeast extract | 2.50 | g | 2.5 | - | |
Na-thioglycolate | 0.50 | g | 0.5 | 4.382 | |
L-Cysteine HCl | 1.00 | g | 1 | 6.344 | |
L-Asparagine | 0.25 | g | 0.25 | 1.892 | |
Glucose | 2.00 | g | 2 | 11.101 | |
Agar | 3.00 | g | 3 | - | |
Distilled water | 1000.00 | ml | - | - | |
1 Boil for 5 min, cool to 50°C while gassing with 80% N2 + 20% CO2. Distribute to anaerobic tubes under the same atmosphere and autoclave. |
909 | TREPONEMA DENTICOLA MEDIUM |