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Main sol. 923

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Internal Solution-ID: S1898

Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na glutamate
(Sigma) 		10.000 g 10 59.133
Yeast extract
(Oxoid) 		5.000 g 5 -
Na2CO3 10.000 g 10 94.35
K2HPO4 0.150 g 0.15 0.861
MgSO4 x 7 H2O 0.025 g 0.025 0.101
(NH4)2SO4 1.000 g 1 7.568
Distilled water 1000.000 ml - -
Agar , for solid medium 20.000 g 20 59.465
1 We recommend that liquid media be made by autoclaving the Na2CO3 separately, otherwise the high pH will cause ammonia to be lost. When making media solidified with agar (20 g/l) the agar should also not be autoclaved with the Na2CO3 otherwise the agar will be hydrolysed. When mixing the ingredients for solid media cool all components to about 55°C otherwise hydrolysis of the agar may occur.
923 ALKALIBACTERIUM OLIVAPOVLITICUS