Main sol. 927
Internal Solution-ID: S1909
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 270.00 | g | 270 | 4620.12 | |
| NaBr | 0.10 | g | 0.1 | 0.972 | |
| MgSO4 x 7 H2O | 20.00 | g | 20 | 81.144 | |
| KCl | 5.00 | g | 5 | 67.069 | |
| NH4Cl | 2.00 | g | 2 | 37.39 | |
| Tris-HCl | 12.00 | g | 12 | - | |
|
Yeast extract
(Difco) |
1.00 | g | 1 | - | |
| Glucose | 2.00 | g | 2 | 11.101 | |
| Distilled water | 1000.00 | ml | - | - | |
| KH2PO4 solution 5%(w/v) | 2.50 | ml | - | - | |
| CaCl2 solution 10%(w/v) | 0.50 | ml | - | - | |
| FeCl2/MnCl2 solution | 0.25 | ml | - | - | |
| 1 Prepare the medium, and adjust the pH to 7.6 with 5M NaOH. | |||||
| 2 Autoclave and when the medium has cooled add 2.5 ml of the phosphate solution, 0.5 ml of the calcium chloride solution, and 0.25 ml of the Fe/Mn solution. This strain will not grow in normal media for halobacteria, such as DSM medium 372! | |||||
Media with this solution
| 927 | HALORHABDUS UTAHENSIS MEDIUM |