Main sol. 930
Internal Solution-ID: S1919
Equipment needed: Filter
Autoclave
Anaerobic workbench
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.20 | g | 0.2 | 1.47 | |
| MgCl2 x 6 H2O | 0.10 | g | 0.1 | 0.492 | |
| NH4Cl | 1.00 | g | 1 | 18.695 | |
| KCl | 0.20 | g | 0.2 | 2.683 | |
| NaCl | 80.00 | g | 80 | 1368.93 | |
| Na2CO3 | 6.83 | g | 6.83 | 64.441 | |
| NaHCO3 | 3.83 | g | 3.83 | 45.591 | |
| Na2S x 9 H2O | 0.20 | g | 0.2 | 0.833 | |
| Yeast extract | 0.20 | g | 0.2 | - | |
| Histidine | 5.00 | g | 5 | 32.227 | |
| Wolin's vitamin solution | 2.00 | ml | - | - | |
| Modified Wolin's mineral solution II | 1.00 | ml | - | - | |
| Resazurin | 1.00 | mg | 1.0e-3 | 0.004 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Final pH 8.9 | |||||
| 2 Prepare the medium without adding the vitamins, histidine, yeast extract, NH4Cl, Na2CO3, and NaHCO3 using anaerobic conditions, under nitrogen. If the medium has been boiled to remove oxygen add the NH4Cl, Na2CO3, and NaHCO3 after the medium had cooled. Dispense into tubes stopper with rubber stoppers (serum tubes or bottles, or screw capped tubes). Autoclave, and to the cooled medium add the vitamins (filter sterilised under N2), histidine, yeast extract, and Na2S x 9 H2O from anaerobic, sterile stock solutions. | |||||
Media with this solution
| 930 | NATRONINCOLA HISTIDINOVORANS MEDIUM |