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Main sol. 934
Internal Solution-ID: S1923
Equipment needed: Anaerobic workbench
Compound
Amount
Unit
Conc. [g/L]
Conc. [mM]
NaCl
1.500
g
1.5
25.667
Na2 SO4
4.000
g
4
28.161
KCl
0.700
g
0.7
9.39
NaHCO3
0.200
g
0.2
2.381
KBr
0.100
g
0.1
0.84
H3 BO3
0.030
g
0.03
0.485
MgCl2
5.800
g
5.8
60.918
CaCl2
1.500
g
1.5
13.516
SrCl2
0.025
g
0.025
0.158
Tryptone
5.000
g
5
70.343
Yeast extract
1.000
g
1
-
Na2 S x 9 H2 O
0.750
g
0.75
3.123
Resazurin
2.000
mg
0.002
0.009
Sulphur
10.000
g
10
-
Distilled water
1000.000
ml
-
-
1
pH 7.0
2
Prepare the medium anaerobically under N2 . Steam the medium for 3 hours on three successive days.
934
THERMOCOCCUS WAIOTAPUENSE MEDIUM
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